It is mostly distributed in China, Japan, Korea and Vietnam. glauca) is a shrub or small tree which can grow up to 8 m. Lindera glauca (Siebold et Zucc.) Blume ( L. Lindera plants have found their various uses as ornamental plants or herbal medicines. The genus Lindera belongs to Lauraceae family and contains more than 100 species that are widely spread in tropical and subtropical areas throughout the world. This essential oil indicated low potential as natural antioxidant, but it could be potentially used as cholinesterase inhibitor with possible application in food, aromatherapy and pharmaceutical industries. Six compounds including 3-carene, limonene, eucalyptol, (E)-β-ocimene, geranyl acetate and β-caryophyllene could contribute together to cholinesterase inhibitory activities of LGFEO. Molecular docking revealed that geranyl acetate, β-caryophyllene and limonene had lower binding affinities in the range of −7.1 to −6.1 kcal/mol through hydrophobic interactions and hydrogen bond. IC 50 values for acetylcholinesterase and butyrylcholinesterase were 46.48 and 34.85 μg/mL, respectively. LGFEO showed obvious cholinesterase inhibitory activities at the final concentrations of 50 and 20 μg/mL. Weak antioxidant activities of LGFEO in three assays (9.52, 11.36 and 38.98 μmol TE/g, respectively) were observed. A total of 48 components were identified representing 95.74% of the total composition of LGFEO in which the major compounds were (E)-β-ocimene (41.53%), α-copaene (13.17%), δ-cadinene (6.20%), 3-carene (5.89%) and eucalyptol (3.57%).

Acetylcholinesterase and butyrylcholinesterase inhibitory activities of LGFEO were tested.

Antioxidant activities of LGFEO were evaluated by three methods with different mechanisms. The LGFEO was extracted by hydro distillation and analyzed by GC-MS and GC-FID. The study on its chemical composition, and antioxidant and cholinesterase inhibitory activities were performed, along with molecular docking of six selected compounds. However, reports on the biological activities of Lindera glauca fruit essential oil (LGFEO) are limited. Lindera glauca is a shrub or small tree mostly distributed in China, Japan and Korea.

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